| # | Name | Relationship/President | Born | Took Office | Left Office | Died |
| 1 | Martha Washington | Wife of George Washington | 06/13/1731 | 04/30/1789 | 03/04/1797 | 05/22/1802 |
| 2 | Abigail Smith Adams | Wife of John Adams | 11/22/1744 | 03/04/1797 | 03/04/1801 | 10/28/1818 |
| 3 | Martha Jefferson Randolph | Daughter of Thomas Jefferson | 09/27/1772 | 03/04/1801 | 03/04/1809 | 10/10/1836 |
| 4 | Dolley Payne Todd Madison | Wife of James Madison | 05/20/1768 | 03/04/1809 | 03/04/1817 | 07/12/1849 |
| 5 | Elizabeth Kortright Monroe | Wife of James Monroe | 06/30/1768 | 03/04/1817 | 03/04/1825 | 09/23/1830 |
| 6 | Louisa Catherine Johnson Adams | Wife of John Quincy Adams | 02/12/1775 | 03/04/1825 | 03/04/1829 | 05/15/1852 |
| 7 | Emily Donelson | Niece of Andrew Jackson | 06/01/1807 | 03/04/1829 | 11/26/1834 | 12/19/1836 |
| 8 | Sarah Yorke Jackson | Daughter-in-law of Andrew Jackson | 07/16/1803 | 11/26/1834 | 03/04/1837 | 08/23/1887 |
| vacant | Martin van Buren, widower | 03/04/1837 | 11/27/1838 | |||
| 9 | Sarah Angelica Singleton | Daughter-in-law of Martin van Buren | 02/13/1818 | 11/27/1838 | 03/04/1841 | 12/29/1877 |
| 10 | Anna Tuthill Symmes Harrison | Wife of William Henry Harrison | 07/25/1775 | 03/04/1841 | 04/04/1841 | 02/25/1864 |
| 11 | Jane Irwin Harrison | Daughter-in-law of William Henry Harrison | 07/23/1804 | 03/04/1841 | 04/04/1841 | 05/11/1846 |
| 12 | Letitia Christian Tyler | Wife of John Tyler | 11/12/1790 | 04/04/1841 | 09/10/1842 | 09/10/1842 |
| 13 | Elizabeth Priscilla Cooper | Daughter-in-law of John Tyler | 06/14/1816 | 09/10/1842 | 06/26/1844 | 12/29/1889 |
| 14 | Julia Gardiner Tyler | Second wife of John Tyler | 05/04/1820 | 06/26/1844 | 03/04/1845 | 07/10/1889 |
| 15 | Sarah Childress Polk | Wife of James K. Polk | 09/04/1803 | 03/04/1845 | 03/04/1849 | 08/14/1891 |
| 16 | Margaret Mackall Smith Taylor | Wife of Zachary Taylor | 09/21/1788 | 03/04/1849 | 07/09/1850 | 08/14/1852 |
| 17 | Abigail Powers Fillmore | Wife of Millard Fillmore | 04/13/1798 | 07/09/1850 | 03/04/1853 | 03/30/1853 |
| 18 | Jane Means Appleton Pierce | Wife of Franklin Pierce | 03/12/1806 | 03/04/1853 | 03/04/1857 | 12/02/1863 |
| 19 | Harriet Rebecca Lane | Niece of James Buchanan | 05/09/1830 | 03/04/1857 | 03/04/1861 | 07/08/1903 |
| 20 | Mary Todd Lincoln | Wife of Abraham Lincoln | 12/13/1818 | 03/04/1861 | 04/15/1865 | 07/16/1882 |
| 21 | Eliza McCardle Johnson | Wife of Andrew Johnson | 10/04/1810 | 04/15/1865 | 03/04/1869 | 01/15/1876 |
| 22 | Julia Boggs Dent Grant | Wife of Ulysses S. Grant | 01/26/1826 | 03/04/1869 | 03/04/1877 | 12/14/1902 |
| 23 | Lucy Ware Webb Hayes | Wife of Rutherford B. Hayes | 08/28/1831 | 03/04/1877 | 03/04/1881 | 06/25/1889 |
| 24 | Lucretia Rudolph Garfield | Wife of James Garfield | 04/19/1832 | 03/04/1881 | 09/19/1881 | 03/14/1918 |
| 25 | Mary Arthur McElroy | Sister of Chester Alan Arthur | 07/05/1841 | 09/19/1881 | 03/04/1885 | 01/08/1917 |
| 26 | Rose Elizabeth Cleveland | Sister of Grover Cleveland | 06/13/1846 | 03/04/1885 | 06/02/1886 | 11/22/1918 |
| 27 | Frances Clara Folsom Cleveland | Wife of Grover Cleveland | 07/21/1864 | 06/02/1886 | 03/04/1889 | 10/29/1947 |
| 28 | Caroline Lavina Scott Harrison | Wife of Benjamin Harrison | 10/01/1832 | 03/04/1889 | 10/25/1892 | 10/25/1892 |
| 29 | Mary Scott Harrison | Daughter of Benjamin Harrison | 04/03/1858 | 10/25/1892 | 03/04/1893 | 10/28/1930 |
| 30 | Ida Saxton McKinley | Wife of William McKinley | 06/08/1847 | 03/04/1893 | 09/14/1901 | 05/26/1907 |
| 31 | Edith Kermit Carow Roosevelt | Wife of Theodore Roosevelt | 08/06/1861 | 09/14/1901 | 03/04/1909 | 09/30/1948 |
| 32 | Helen Louise Herron Taft | Wife of William Howard Taft | 06/02/1861 | 03/04/1909 | 03/04/1913 | 05/22/1943 |
| 33 | Ellen Louise Axson Wilson | Wife of Woodrow Wilson | 05/15/1860 | 03/04/1913 | 08/06/1914 | 08/06/1914 |
| 34 | Margaret Woodrow Wilson | Daughter of Woodrow Wilson | 04/16/1886 | 08/06/1914 | 12/18/1915 | 02/12/1944 |
| 35 | Edith Bolling Galt Wilson | Second wife of Woodrow Wilson | 10/15/1872 | 12/18/1915 | 03/04/1921 | 12/28/1961 |
| 36 | Florence Kling Harding | Wife of Warren G. Harding | 08/15/1860 | 03/04/1921 | 08/02/1923 | 11/21/1924 |
| 37 | Grace Anna Goodhue Coolidge | Wife of Calvin Coolidge | 01/03/1879 | 08/02/1923 | 03/04/1929 | 07/08/1957 |
| 38 | Lou Henry Hoover | Wife of Herbert Hoover | 03/29/1874 | 03/04/1929 | 03/04/1933 | 01/07/1944 |
| 39 | Anna Eleanor Roosevelt | Wife of Franklin Delano Roosevelt | 10/11/1884 | 03/04/1933 | 04/12/1945 | 11/07/1962 |
| 40 | Elizabeth Virginia "Bess" Wallace Truman | Wife of Harry S. Truman | 02/13/1885 | 04/12/1945 | 01/20/1953 | 10/18/1982 |
| 41 | Mamie Geneva Doud Eisenhower | Wife of Dwight David Eisenhower | 11/14/1896 | 01/20/1953 | 01/20/1961 | 11/01/1979 |
| 42 | Jacqueline Bouvier Kennedy (Onassis) | Wife of John F. Kennedy | 07/28/1929 | 01/20/1961 | 11/22/1963 | 05/19/1994 |
| 43 | Claudia Alta "Lady Bird" Taylor Johnson | Wife of Lyndon Baines Johnson | 12/22/1912 | 11/22/1963 | 01/20/1969 | 07/11/2007 |
| 44 | Thelma "Pat" Catherine Ryan Nixon | Wife of Richard Milhous Nixon | 03/16/1912 | 01/20/1969 | 08/09/1974 | 06/22/1993 |
| 45 | Elizabeth Ann Bloomer Ford | Wife of Gerald Rudolph Ford | 04/08/1918 | 08/09/1974 | 01/20/1977 | 07/08/2011 |
| 46 | Rosalynn Carter | Wife of Jimmy Carter | 08/18/1927 | 01/20/1977 | 01/20/1981 | |
| 47 | Nancy Davis Reagan | Wife of Ronald Wilson Reagan | 07/06/1921 | 01/20/1981 | 01/20/1989 | 03/06/2016 |
| 48 | Barbara Bush | Wife of George Herbert Walker Bush | 06/08/1925 | 01/20/1989 | 01/20/1993 | 04/17/2018 |
| 49 | Hillary Rodham Clinton | Wife of William Jefferson "Bill" Clinton | 10/26/1947 | 01/20/1993 | 01/20/2001 | |
| 50 | Laura Welch Bush | Wife of George W. Bush | 11/04/1946 | 01/20/2001 | 01/20/2009 | |
| 51 | Michelle LaVaughn Robinson Obama | Wife of Barack Obama | 01/17/1964 | 01/20/2009 | 01/20/2017 | |
| 52 | vacant | No legally elected president | 01/20/2017 | 01/20/2021 | ||
| 53 | Jill Biden | Wife of Joe Biden | 06/03/1951 | 01/20/2021 | ||
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